3 ECTS credits
78 h study time

Offer 1 with catalog number 4021498ENR for all students in the 1st semester at a (E) Master - advanced level.

Semester
1st semester
Enrollment based on exam contract
Impossible
Grading method
Grading (scale from 0 to 20)
Can retake in second session
Yes
Taught in
English
Partnership Agreement
Under interuniversity agreement for degree program
Faculty
Faculty of Sciences and Bioengineering Sciences
Department
Bio-Engineering Sciences
Educational team
Jo Van Ginderachter
Nick Devoogdt
Steven Ballet (course titular)
Activities and contact hours

26 contact hours Lecture
Course Content

Part Combinatorial Chemistry

The next topics are discussed:

  • Solution Phase Combinatorial Synthesis: Parallel Synthesis, One-pot Synthesis, Scavengers, Quenchers, Resin bound reagents, Solution Phase Combinatorial Chemistry using Dendrimers, Classical purification by extraction, Fluorous phase chemistry.
  • Solid Phase Combinatorial Synthesis: Resins, Linkers, Solution Phase Synthesis versus Solid Phase Synthesis.
  • Mixed versus Parallel Synthesis,
  • Identification of the Active Component (Mix-and-split Synthesis, Micro Analysis, Off-bead Mass Spectrometry, On-bead Mass Spectrometry, On-bead analysis with NMR-Spectroscopy, Infrared Spectrometry, Iterative Deconvolution, Recursive Method, Positional Scanning), Encoding of Libraries (Chemical Tagging, Binary Encoding, Radio Frequency (RF) Markers, Laser (optical) Encoding, Fluorescence Encoding),
  • Dynamic combinatorial chemistry
  • Varying case studies

Part of Combinatorial BIO-chemistry:


In this part we review:

  • the construction and screening of genomic and cDNA banks.
  • the selection of peptide libraries via phage-display, introducing M13 gene3 containing phasmids, selection procedures and providing solutions to remediate the reduced performance of virions.
  • the alternative non-phage display systems and screenings, including in vitro selection systems such as SELEX, ribosome display, RNA peptide fusion and compartimentalised systems.
  • the selection techniques for functional entities and in vitro transcription/translation and protein interactions on µ-arrays and the generation of new man-made proteins.
  • Techniques to determine cellular characteristics via fluorescence (immunohistochemistry, fluorescence microscopy, flow cytometry)
  • Mass cytometry of cells
  • Techniques to determine the epigenome of cells (ATAC-seq, ChIP-seq)
  • Techniques to determine the transcriptome of individual cells (single cell RNA-seq)
Additional info

None

Learning Outcomes

General competences

Part Combinatorial Chemistry
The student

  • understands the principles of combinatorial chemistry, both in solution and on solid phase, and their application in medicinal chemistry and the pharmaceutical industry.
  • understands the chemical reactivity of the different conversions used during this course
  • is acquainted with (high-throughput) techniques (synthetic and analytical) used in combinatorial chemistry and knows their advantages and disadvantages.


Part of Combinatorial BIO-chemistry

The student understands

  • the various techniques used to construct immense large combinatorial libraries of bio-molecules
  • their screening in a high-throughput approach for functional biochemical molecules
  • the techniques to determine characteristics of individual cells, at the level of proteins, epigenome and transcriptome

Grading

The final grade is composed based on the following categories:
Other Exam determines 100% of the final mark.

Within the Other Exam category, the following assignments need to be completed:

  • Other exam with a relative weight of 1 which comprises 100% of the final mark.

Additional info regarding evaluation

Part Combinatorial Chemistry (Prof S. Ballet)
Oral exam with written preparation and written exam. Knowledge and insight of the student are tested. 

Part of Combinatorial BIO-chemistry (Prof Van Ginderachter/Devoogdt)
Oral examination with written preparation.

The final marks are determined as follows: Combinatorial Chemistry (Prof Ballet): 3/6; BIO-chemistry (Prof Devoogdt): 2/6; BIO-chemistry (Prof Van Ginderachter): 1/6. 

If the student obtains at least half of the score for an individual part (Combinatorial chemistry or BIO-chemistry), partial marks are transferred to the second session and to the next academic year. Students may not relinquish partial marks.

Allowed unsatisfactory mark
The supplementary Teaching and Examination Regulations of your faculty stipulate whether an allowed unsatisfactory mark for this programme unit is permitted.

Academic context

This offer is part of the following study plans:
Master of Molecular Biology: Standaard traject