5 ECTS credits
150 h study time
Offer 1 with catalog number 4021483DNR for all students in the 1st semester at a (D) Master - preliminary level.
Biosafety rules in the lab
Proper pipetting technique
Making solutions : familiarization with molarity, buffers and basic calculations
Using common instruments in the lab (balance, pH-meters, centrifuge)
DNA purification from plant material
Electrophoretic methods for proteins and nucleic acids
Protein and DNA concentration determination
Restriction endonucleases digestion
Bacterial culture
Plasmid isolation
Competent cells preparation and transformation
Polymerase Chain Reaction (PCR) (amplification of a known gene fragment)
Protein concentration determination methods
Enzyme-Linked ImmunoSorbent Assay (ELISA)
SDS-PAGE, Western blot
Expression vectors
Recombinant protein purification from expression host
None
Students have developed practical skills to work in a safe way in the laboratory, according to good laboratory practices.
Students understand current laboratory techniques and can plan and execute molecular biology experiments; they can interpret and report the results of their experiments.
The final grade is composed based on the following categories:
Other Exam determines 100% of the final mark.
Within the Other Exam category, the following assignments need to be completed:
Evaluation is based on the performance, punctuality, motivation and attitude of the student in the labs, as well as on the reporting.
This offer is part of the following study plans:
Master of Molecular Biology: Standaard traject