3 ECTS credits
90 h study time
Offer 1 with catalog number 1010269BNR for all students in the 1st semester at a (B) Bachelor - advanced level.
In this course the following items are discussed: (1) the enzymes (restriction enzymes, DNA and RNA polymerases, DNA and RNA ligases, phosphatases, kinases, transferases, nucleases), (2) different detection techniques, (3) the use and synthesis of synthetic primers (primer design, modifications), (4) the DNA sequencing techniques, (5) PCR technology, (6) Tn-seq, EMSA and CRISPR/Cas9 methods, (7) cloning vectors, (8) expression vectors in prokaryotes (promoters, plasmid copy number en stability, integration in the genome, codon usage, fusion proteins and tags) and in eukaryotes such as yeast (S. cerevisiae, P. pastoris), insect cells (Baculovirus) and animal cells, (9) transgenic animals and plants and (10) reverse genetics in bacteria (transposon mutagenesis, site-specific mutagenesis, site-directed mutagenesis), plants and animals.
Students can contact Charles Van der Henst by email (charles.van.der.henst@vub.be). Power point presentation of the course Gene technology
Complementary study material:
References are communicated (books) to or copies (articles) are given to the students.
Objectives
The students will learn the techniques used in molecular biology to analyse and manipulate nucleic acids. The use of gene technology in fundamental research and application in industry will be illustrated.
Endterms
To make the students familiar with the different techniques used in molecular biology and biotechnology.
The final grade is composed based on the following categories:
Written Exam determines 100% of the final mark.
Within the Written Exam category, the following assignments need to be completed:
Oral examen on the content of the course.
The student should have a fundamental understanding of gene technology and the applications of this technology.
This offer is part of the following study plans:
Bachelor of Biology: Default track (only offered in Dutch)
Bachelor of Bioengineering Sciences: Profile Cell and Gene Biotechnology (only offered in Dutch)
Bachelor of Bioengineering Sciences: Profile Chemistry and Bioprocess Technology (only offered in Dutch)
Bachelor of Bioengineering Sciences: Initial track (only offered in Dutch)
Bridging Programme Master of Science in Biology: Standaard (53 SP) (only offered in Dutch)