3 ECTS credits
90 h study time

Offer 1 with catalog number 1010269BNR for all students in the 1st semester at a (B) Bachelor - advanced level.

Semester
1st semester
Enrollment based on exam contract
Impossible
Grading method
Grading (scale from 0 to 20)
Can retake in second session
Yes
Enrollment Requirements
Students who want to enroll for this course, must have passed for “Biochemistry” and ''General and Molecular Genetics including Bio-Ethics Seminars". Students Bio-Engineering should additionally have acquired 33 ECTS in the module “Basis Sciences”.
Taught in
Dutch
Faculty
Faculty of Sciences and Bioengineering Sciences
Department
Bio-Engineering Sciences
Educational team
Charles Van der Henst (course titular)
Activities and contact hours

26 contact hours Lecture
Course Content

In this course the following items are discussed: (1) the enzymes (restriction enzymes, DNA and RNA polymerases, DNA and RNA ligases, phosphatases, kinases, transferases, nucleases), (2) different detection techniques, (3) the use and synthesis of synthetic primers (primer design, modifications), (4) the DNA sequencing techniques, (5) PCR technology, (6) Tn-seq, EMSA and CRISPR/Cas9 methods, (7) cloning vectors, (8) expression vectors in prokaryotes (promoters, plasmid copy number en stability, integration in the genome, codon usage, fusion proteins and tags) and in eukaryotes such as yeast (S. cerevisiae, P. pastoris), insect cells (Baculovirus) and animal cells, (9) transgenic animals and plants and (10) reverse genetics in bacteria (transposon mutagenesis, site-specific mutagenesis, site-directed mutagenesis), plants and animals.

Course material
Digital course material (Required) : Uitgewerkte cursus Gentechnologie
Practical course material (Required) : Referentiewerken (boeken, artikels) worden medegedeeld en / of overhandigd aan de studenten
Additional info

Students can contact Charles Van der Henst by email (charles.van.der.henst@vub.be). Power point presentation of the course Gene technology

Complementary study material:
References are communicated (books) to or copies (articles) are given to the students.

Learning Outcomes

General competencies

Objectives
The students will learn the techniques used in molecular biology to analyse and manipulate nucleic acids. The use of gene technology in fundamental research and application in industry will be illustrated.

Endterms
To make the students familiar with the different techniques used in molecular biology and biotechnology.

Grading

The final grade is composed based on the following categories:
Written Exam determines 100% of the final mark.

Within the Written Exam category, the following assignments need to be completed:

  • Schriftelijk examen with a relative weight of 1 which comprises 100% of the final mark.

    Note: Schriftelijk examen over de inhoud van de cursus.
    Verwacht wordt dat de student een goede basis kennis heeft van de gentechnologie en de toepassingen van deze technologie.

Additional info regarding evaluation

Oral examen on the content of the course.
The student should have a fundamental understanding of gene technology and the applications of this technology.

Allowed unsatisfactory mark
The supplementary Teaching and Examination Regulations of your faculty stipulate whether an allowed unsatisfactory mark for this programme unit is permitted.

Academic context

This offer is part of the following study plans:
Bachelor of Biology: Default track (only offered in Dutch)
Bachelor of Bioengineering Sciences: Profile Cell and Gene Biotechnology (only offered in Dutch)
Bachelor of Bioengineering Sciences: Profile Chemistry and Bioprocess Technology (only offered in Dutch)
Bachelor of Bioengineering Sciences: Initial track (only offered in Dutch)
Bridging Programme Master of Science in Biology: Standaard (53 SP) (only offered in Dutch)