3 ECTS credits
80 h study time

Offer 1 with catalog number 4005244FNR for all students in the 1st semester at a (F) Master - specialised level.

Semester
1st semester
Enrollment based on exam contract
Impossible
Grading method
Grading (scale from 0 to 20)
Can retake in second session
Yes
Enrollment Requirements
Students must have followed ‘Biophysical Chemistry’, before they can enroll for ‘Protein Engineering’. Enrolling in ‘Protein Engineering’ means that you simultaneously follow 'Protein Chemistry, function and structure' or have successfully passed ‘'Protein Chemistry, function and structure’.
Taught in
Dutch
Faculty
Faculty of Science and Bio-engineering Sciences
Department
Bio-Engineering Sciences
Educational team
Jan Steyaert (course titular)
Activities and contact hours
26 contact hours Lecture
Course Content

 

This course aims at giving an overview of several methods to produce non-natural proteins and the uses thereof, often referred to as protein engineering. The course includes the following topics:

(1) Guidelines for functional analysis of engineered and mutant enzymes
(2) phi-value analysis of protein folding and stability
(3) engineering enantioselectivity
(4) Phage display systems for protein engineering
(5) Cell-free display systems for protein engineering
(6) library construction for protein engineering
(7) Design and engineering of synthetic binding proteins
(8) combinatorial enzyme engineering
(9) engineering of therapeutic proteins
(10) Inteins and protein cyclisation
(11) engineering using noncanonical amino acids
(12) Computer modeling, homology modeling and bioinformatics
(13) Knowledge based protein design
(14) from prospecting to product: metagenomics
(15) Modulating protein structure
(16) Modulating intrinsic properties of proteins
(17) designing protein switches
(18) chimeragenesis in protein engineering
(19) patway engineering
(20) silk proteins and biomaterials

Course material
Digital course material (Required) : Slides
Handbook (Recommended) : Protein Engineering Handbook, 2-Volume Set, Stefan Lutz (Editor), Uwe Theo Bornscheuer (Editor), Wiley, VCH, 9780470060988, 2008
Handbook (Recommended) : Protein Engineering and Design, Sheldon J. Park (Editor), Jennifer R. Cochran (Editor), CRC press, 9781420076585, 2009
Additional info

The course is given by using transparancies and handouts

Complementary study material:

Aanvullend studiemateriaal:
Protein Engineering Handbook (2-Volume Set) [Hardcover]
Stefan Lutz (Editor), Uwe Theo Bornscheuer (Editor)
Wiley, VCH

Protein Engineering and Design [Hardcover]
Sheldon J. Park (Editor), Jennifer R. Cochran (Editor)
CRC press

Learning Outcomes

General competencies

By using protein engineering, one can design new or modify exsisting proteins with new functions. The protein engineering course aims to introduce the available technologies to the students.



After the course the students:

- can describe the protein engineering cycle

- know the basic techniques

- know how to use protein engineering techniques to study aspects of catalysis, folding and stability

- have studied examples of rationale proten design

- Know the basic principles of antibody engineering

- have studied applications of protein engineering in agriculture, industry and medicine

Grading

The final grade is composed based on the following categories:
Oral Exam determines 100% of the final mark.

Within the Oral Exam category, the following assignments need to be completed:

  • Mondeling examen with a relative weight of 1 which comprises 100% of the final mark.

Additional info regarding evaluation

Oral examination folloing a written preparation fase


Examples of questions:

1) Describe the principle of phage display
2) Explain the mechanism of inteins and two applications thereof

Allowed unsatisfactory mark
The supplementary Teaching and Examination Regulations of your faculty stipulate whether an allowed unsatisfactory mark for this programme unit is permitted.

Academic context

This offer is part of the following study plans:
Master of Bioengineering Sciences: Cell and Gene Biotechnology: Medical Biotechnology (only offered in Dutch)
Master of Bioengineering Sciences: Cell and Gene Biotechnology: Molecular Biotechnology (only offered in Dutch)
Master of Bioengineering Sciences: Cell and Gene Biotechnology: Agrobiotechnology (only offered in Dutch)
Master of Bioengineering Sciences: Chemistry and Bioprocess Technology: Food Biotechnology (only offered in Dutch)
Master of Bioengineering Sciences: Chemistry and Bioprocess Technology: Chemical Biotechnology (only offered in Dutch)
Master of Bioengineering Sciences: Chemistry and Bioprocess Technology: Biochemical Biotechnology (only offered in Dutch)