3 ECTS credits
78 u studietijd
Aanbieding 1 met studiegidsnummer 4021498ENR voor alle studenten in het 1e semester
met een verdiepend master niveau.
- Semester
- 1e semester
- Inschrijving onder examencontract
- Niet mogelijk
- Beoordelingsvoet
- Beoordeling (0 tot 20)
- 2e zittijd mogelijk
- Ja
- Onderwijstaal
- Engels
- Onder samenwerkingsakkoord
- Onder interuniversitair akkoord mbt. opleiding
- Faculteit
- Faculteit Wetenschappen en Bio-ingenieurswetensch.
- Verantwoordelijke vakgroep
- Bio-ingenieurswetenschappen
- Onderwijsteam
- Jo Van Ginderachter
Nick Devoogdt
Steven Ballet
(titularis)
- Onderdelen en contacturen
- 26 contacturen Hoorcollege
- Inhoud
Part Combinatorial Chemistry
The next topics are discussed:
- Solution Phase Combinatorial Synthesis: Parallel Synthesis, One-pot Synthesis, Scavengers, Quenchers, Resin bound reagents, Solution Phase Combinatorial Chemistry using Dendrimers, Classical purification by extraction, Fluorous phase chemistry.
- Solid Phase Combinatorial Synthesis: Resins, Linkers, Solution Phase Synthesis versus Solid Phase Synthesis.
- Mixed versus Parallel Synthesis,
- Identification of the Active Component (Mix-and-split Synthesis, Micro Analysis, Off-bead Mass Spectrometry, On-bead Mass Spectrometry, On-bead analysis with NMR-Spectroscopy, Infrared Spectrometry, Iterative Deconvolution, Recursive Method, Positional Scanning), Encoding of Libraries (Chemical Tagging, Binary Encoding, Radio Frequency (RF) Markers, Laser (optical) Encoding, Fluorescence Encoding),
- Dynamic combinatorial chemistry
- Varying case studies
Part of Combinatorial BIO-chemistry:
In this part we review:
- the construction and screening of genomic and cDNA banks.
- the selection of peptide libraries via phage-display, introducing M13 gene3 containing phasmids, selection procedures and providing solutions to remediate the reduced performance of virions.
- the alternative non-phage display systems and screenings, including in vitro selection systems such as SELEX, ribosome display, RNA peptide fusion and compartimentalised systems.
- the selection techniques for functional entities and in vitro transcription/translation and protein interactions on µ-arrays and the generation of new man-made proteins.
- Techniques to determine cellular characteristics via fluorescence (immunohistochemistry, fluorescence microscopy, flow cytometry)
- Mass cytometry of cells
- Techniques to determine the epigenome of cells (ATAC-seq, ChIP-seq)
- Techniques to determine the transcriptome of individual cells (single cell RNA-seq)
- Bijkomende info
None
- Leerresultaten
-
General competences
Part Combinatorial Chemistry
The student
- understands the principles of combinatorial chemistry, both in solution and on solid phase, and their application in medicinal chemistry and the pharmaceutical industry.
- understands the chemical reactivity of the different conversions used during this course
- is acquainted with (high-throughput) techniques (synthetic and analytical) used in combinatorial chemistry and knows their advantages and disadvantages.
Part of Combinatorial BIO-chemistry
The student understands
- the various techniques used to construct immense large combinatorial libraries of bio-molecules
- their screening in a high-throughput approach for functional biochemical molecules
- the techniques to determine characteristics of individual cells, at the level of proteins, epigenome and transcriptome
- Beoordelingsinformatie
-
De beoordeling bestaat uit volgende opdrachtcategorieën:
Examen Andere bepaalt 100% van het eindcijfer
Binnen de categorie Examen Andere dient men volgende opdrachten af te werken:
- Examen andere
met een wegingsfactor 1
en aldus 100% van het totale eindcijfer.
- Aanvullende info mbt evaluatie
Part Combinatorial Chemistry (Prof S. Ballet)
Oral exam with written preparation and written exam. Knowledge and insight of the student are tested.
Part of Combinatorial BIO-chemistry (Prof Van Ginderachter/Devoogdt)
Oral examination with written preparation.
The final marks are determined as follows: Combinatorial Chemistry (Prof Ballet): 3/6; BIO-chemistry (Prof Devoogdt): 2/6; BIO-chemistry (Prof Van Ginderachter): 1/6.
If the student obtains at least half of the score for an individual part (Combinatorial chemistry or BIO-chemistry), partial marks are transferred to the second session and to the next academic year. Students may not relinquish partial marks.
- Toegestane onvoldoende
- Kijk in het aanvullend OER van je faculteit na of een toegestane onvoldoende mogelijk is voor dit opleidingsonderdeel.
Academische context
Deze aanbieding maakt deel uit van de volgende studieplannen:
Master in de biomedische wetenschappen: management en bedrijfsleven
Master in de biomedische wetenschappen: klinische biomedische wetenschappen
Master of Molecular Biology: Standaard traject (enkel aangeboden in het Engels)