3 ECTS credits
78 u studietijd

Aanbieding 1 met studiegidsnummer 4021498ENR voor alle studenten in het 1e semester met een verdiepend master niveau.

Semester
1e semester
Inschrijving onder examencontract
Niet mogelijk
Beoordelingsvoet
Beoordeling (0 tot 20)
2e zittijd mogelijk
Ja
Onderwijstaal
Engels
Onder samenwerkingsakkoord
Onder interuniversitair akkoord mbt. opleiding
Faculteit
Faculteit Wetenschappen en Bio-ingenieurswetensch.
Verantwoordelijke vakgroep
Bio-ingenieurswetenschappen
Onderwijsteam
Jo Van Ginderachter
Nick Devoogdt
Steven Ballet (titularis)
Onderdelen en contacturen
0 contacturen Exam
26 contacturen Lecture
Inhoud

Part Combinatorial Chemistry

The next topics are discussed:

  • Solution Phase Combinatorial Synthesis: Parallel Synthesis, One-pot Synthesis, Scavengers, Quenchers, Resin bound reagents, Solution Phase Combinatorial Chemistry using Dendrimers, Classical purification by extraction, Fluorous phase chemistry.
  • Solid Phase Combinatorial Synthesis: Resins, Linkers, Solution Phase Synthesis versus Solid Phase Synthesis.
  • Mixed versus Parallel Synthesis,
  • Identification of the Active Component (Mix-and-split Synthesis, Micro Analysis, Off-bead Mass Spectrometry, On-bead Mass Spectrometry, On-bead analysis with NMR-Spectroscopy, Infrared Spectrometry, Iterative Deconvolution, Recursive Method, Positional Scanning), Encoding of Libraries (Chemical Tagging, Binary Encoding, Radio Frequency (RF) Markers, Laser (optical) Encoding, Fluorescence Encoding),
  • Dynamic combinatorial chemistry
  • Varying case studies

Part of Combinatorial BIO-chemistry:


In this part we review:

  • the construction and screening of genomic and cDNA banks.
  • the selection of peptide libraries via phage-display, introducing M13 gene3 containing phasmids, selection procedures and providing solutions to remediate the reduced performance of virions.
  • the alternative non-phage display systems and screenings, including in vitro selection systems such as SELEX, ribosome display, RNA peptide fusion and compartimentalised systems.
  • the selection techniques for functional entities and in vitro transcription/translation and protein interactions on µ-arrays and the generation of new man-made proteins.
  • Techniques to determine cellular characteristics via fluorescence (immunohistochemistry, fluorescence microscopy, flow cytometry)
  • Mass cytometry of cells
  • Techniques to determine the epigenome of cells (ATAC-seq, ChIP-seq)
  • Techniques to determine the transcriptome of individual cells (single cell RNA-seq)
Bijkomende info

None

Leerresultaten

General competences

Part Combinatorial Chemistry
The student

  • understands the principles of combinatorial chemistry, both in solution and on solid phase, and their application in medicinal chemistry and the pharmaceutical industry.
  • understands the chemical reactivity of the different conversions used during this course
  • is acquainted with (high-throughput) techniques (synthetic and analytical) used in combinatorial chemistry and knows their advantages and disadvantages.


Part of Combinatorial BIO-chemistry

The student understands

  • the various techniques used to construct immense large combinatorial libraries of bio-molecules
  • their screening in a high-throughput approach for functional biochemical molecules
  • the techniques to determine characteristics of individual cells, at the level of proteins, epigenome and transcriptome

Beoordelingsinformatie

De beoordeling bestaat uit volgende opdrachtcategorieën:
Examen Andere bepaalt 100% van het eindcijfer

Binnen de categorie Examen Andere dient men volgende opdrachten af te werken:

  • Examen andere met een wegingsfactor 1 en aldus 100% van het totale eindcijfer.

Aanvullende info mbt evaluatie

Part Combinatorial Chemistry (Prof S. Ballet)
Oral exam with written preparation and written exam. Knowledge and insight of the student are tested. 

Part of Combinatorial BIO-chemistry (Prof Van Ginderachter/Devoogdt)
Oral examination with written preparation.

The final marks are determined as follows: Combinatorial Chemistry (Prof Ballet): 3/6; BIO-chemistry (Prof Devoogdt): 2/6; BIO-chemistry (Prof Van Ginderachter): 1/6. 

If the student obtains at least half of the score for an individual part (Combinatorial chemistry or BIO-chemistry), partial marks are transferred to the second session and to the next academic year. Students may not relinquish partial marks.

Toegestane onvoldoende
Kijk in het aanvullend OER van je faculteit na of een toegestane onvoldoende mogelijk is voor dit opleidingsonderdeel.

Academische context

Deze aanbieding maakt deel uit van de volgende studieplannen:
Master of Molecular Biology: Standaard traject (enkel aangeboden in het Engels)