5 ECTS credits
150 u studietijd
Aanbieding 1 met studiegidsnummer 4021483DNR voor alle studenten in het 1e semester met een inleidend master niveau.
Biosafety rules in the lab
Proper pipetting technique
Making solutions : familiarization with molarity, buffers and basic calculations
Using common instruments in the lab (balance, pH-meters, centrifuge)
DNA purification from plant material
Electrophoretic methods for proteins and nucleic acids
Protein and DNA concentration determination
Restriction endonucleases digestion
Bacterial culture
Plasmid isolation
Competent cells preparation and transformation
Polymerase Chain Reaction (PCR) (amplification of a known gene fragment)
Protein concentration determination methods
Enzyme-Linked ImmunoSorbent Assay (ELISA)
SDS-PAGE, Western blot
Expression vectors
Recombinant protein purification from expression host
None
Students have developed practical skills to work in a safe way in the laboratory, according to good laboratory practices.
Students understand current laboratory techniques and can plan and execute molecular biology experiments; they can interpret and report the results of their experiments.
De beoordeling bestaat uit volgende opdrachtcategorieën:
Examen Andere bepaalt 100% van het eindcijfer
Binnen de categorie Examen Andere dient men volgende opdrachten af te werken:
Evaluation is based on the performance, punctuality, motivation and attitude of the student in the labs, as well as on the reporting.
Deze aanbieding maakt deel uit van de volgende studieplannen:
Master of Molecular Biology: Standaard traject (enkel aangeboden in het Engels)