5 ECTS credits
125 h study time

Offer 1 with catalog number 4017442FNR for all students in the 1st semester at a (F) Master - specialised level.

Semester
1st semester
Enrollment based on exam contract
Impossible
Grading method
Grading (scale from 0 to 20)
Can retake in second session
Yes
Taught in
English
Faculty
Faculty of Sciences and Bioengineering Sciences
Department
Bio-Engineering Sciences
Educational team
Jo Van Ginderachter
Nick Devoogdt
Steven Ballet (course titular)
Activities and contact hours
26 contact hours Lecture
52 contact hours Independent or External Form of Study
Course Content

Part Combinatorial Chemistry

The next topics are discussed:

  • Solution Phase Combinatorial Synthesis: Parallel synthesis, One-pot synthesis, scavengers, quenchers, resin-bound reagents, solution phase combinatorial chemistry using dendrimers, classical purification by extraction, fluorous phase chemistry,
  • Solid Phase Combinatorial Synthesis: Resins, linkers, solution phase synthesis versus solid phase synthesis.
  • Mixed versus parallel synthesis,
  • Identification of the active components (Mix-and-split synthesis, micro analysis, Off-bead mass spectrometry, On-bead mass spectrometry, On-bead analysis with NMR spectroscopy, infrared spectrometry, iterative deconvolution, recursive method, positional scanning), encoding of libraries (chemical tagging, binary encoding, radio frequency (RF) markers, laser (optical) encoding, fluorescence encoding),
  • Dynamic combinatorial chemistry
  • Varying case studies

Part of Combinatorial BIO-chemistry:


In this part we review:

  • the construction and screening of genomic and cDNA banks.
  • the selection of peptide libraries via phage-display, introducing M13 gene3 containing phasmids, selection procedures and providing solutions to remediate the reduced performance of virions.
  • the alternative non-phage display systems and screenings, including in vitro selection systems such as SELEX, ribosome display, RNA peptide fusion and compartimentalised systems.
  • the selection techniques for functional entities and in vitro transcription/translation and protein interactions on µ-arrays and the generation of new man-made proteins.
  • Techniques to determine cellular characteristics via fluorescence (immunohistochemistry, fluorescence microscopy, flow cytometry)
  • Mass cytometry of cells
  • Techniques to determine the epigenome of cells (ATAC-seq, ChIP-seq)
  • Techniques to determine the transcriptome of individual cells (single cell RNA-seq)

 

 

Course material
Digital course material (Required) : Combinatoriële biochemie, Notities en powerpoint presentaties
Course text (Required) : Combinatoriële chemie, Cursustekst, notities en powerpoint presentaties, Door de prof
Course text (Required) : Drug Design, Cursustekst, notities en powerpoint presentaties, Door de prof
Additional info

N/A

Learning Outcomes

General competencies

Part Combinatorial Chemistry

The student

  • understands the principles of combinatorial chemistry, both in solution and on solid phase, and their application in medicinal chemistry and the pharmaceutical industry .
  • understands the reactivity of the different conversions used during this course
  • is acquainted with (high-throughput) techniques (synthetic and analytical) used in combinatorial chemistry and knows their advantages and disadvantages.


Part of Combinatorial BIO-chemistry

The student understands

  • the various techniques used to construct immense large combinatorial libraries of bio-molecules
  • their screening in a high-throughput approach for functional biochemical molecules
  • the techniques to determine characteristics of individual cells, at the level of proteins, epigenome and transcriptome

 

Grading

The final grade is composed based on the following categories:
Oral Exam determines 67% of the final mark.
Written Exam determines 33% of the final mark.

Within the Oral Exam category, the following assignments need to be completed:

  • examen mondeling with a relative weight of 2 which comprises 44.7% of the final mark.

    Note: Partim Combinatoriële BIO-chemie
  • examen mondeling with a relative weight of 1 which comprises 22.3% of the final mark.

    Note: Partim Combinatoriële Chemie

Within the Written Exam category, the following assignments need to be completed:

  • examen schriftelijk with a relative weight of 1 which comprises 33% of the final mark.

    Note: Partim Combinatoriële Chemie

Additional info regarding evaluation

Part Combinatorial Chemistry (Prof S. Ballet)
Oral exam with written preparation and written examination

Part of Combinatorial BIO-chemistry (Prof Van Ginderachter/Devoogdt)
Oral examination with written preparation.

The final marks are the average of the grades obtained for each of the three parts. The student must succeed (10/20) for all three parts in order to pass the complete course.

If the student obtains at least half of the score for an individual part, partial marks are transferred to the second session and to the next academic year. Students may not relinquish partial marks.

 

Allowed unsatisfactory mark
The supplementary Teaching and Examination Regulations of your faculty stipulate whether an allowed unsatisfactory mark for this programme unit is permitted.

Academic context

This offer is part of the following study plans:
Master of Molecular Biology: Standaard traject